CRISPR Guide Enrichment Kit
Every edit, every cell.
Explore CRISPR perturbations at a single cell transcriptome level with Scale Bio’s CRISPR Guide Enrichment Kit.
Benefits of CRISPR Guide Enrichment Kit
Superior
sensitivity
Customizable
library prep
Easily
scalable
Streamlined sample
multiplexing
Key advantages
Superior sensitivity
- Compared to traditional CROP-seq methods utilizing polyA capture, our kit enables much higher guide sensitivity with a custom RT primer
% Reads in Enriched Library Containing a Guide
% Cells with a Guide
Assigned
(CRISPR Guide Enrichment Kit)
Customizable library prep
- No need to change vectors, our library prep is compatible with any CROP-style vector
- Customize your enrichment strategy by selecting either our Cas9 or non-Cas9 kits
Easily scalable
- Run 125,000 cells per experiment, or use our extended throughput plates to capture up to 500,000 cells
Streamlined workflow
CRISPR Guide Enrichment Kit is designed based on the Scale Bio Single Cell RNA Sequencing Kit workflow, but includes guide-specific priming during RT and a guide-specific PCR enrichment step. Compatible with Cas9 and non-Cas9 vectors, the Guide Enrichment Kit offers flexibility for your high-throughput single cell screening.
CRISPR sgRNA library generation – Target genes and pathways of interest or use an off-the shelf CRISPR library
Cell transduction and selection – Transduce cells with guide RNAs at optimized MOI and perform selection
Fixation and library preparation – Fix cells and generate scRNA-seq and paired CRISPR guide libraries using Scale Bio reagents
Sequencing and data analysis – Analyze data with our CRISPR software pipeline
Featured publication
Resources
Frequently asked questions
We do provide a software tool specific to the CRISPR Guide Enrichment Kit.
We have tested compatibility on MiSeq, iSeq, NextSeq 500/550, NextSeq 1000/2000, NovaSeq6000.
Even with a 10-fold increase in throughput, our combinatorial indexing method results in an effective doublet rate of ~4.7%.
Yes, you can access our public data sets at the following link:
Want to learn more about the Single Cell CRISPR Guide Enrichment Kit?
Reach out to our team for more information or to request a price quote.
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