How it works
ScaleBio’s single cell sequencing library preparation technology and single-cell analysis solutions enable unprecedented sample multiplexing, cell throughput, and multi-omics profiling of cells across a broad range of genomic, epigenomic, transcriptomic, proteogenomic, and multi-omic applications.
Split-pool barcoding and combinatorial indexing methods leverage the cell as a compartment for a simple, instrument-free single-cell workflow as follows:
1. Permeabilized cells are distributed in a multi-well plate where barcoded probes tag cellular analytes with a barcode that is uniform for all cells within a single well.
2. These barcoded cells are pooled, then randomly re-distributed for another round of barcoding.
3. This process is completed when the desired barcode space has been achieved. The number of cells profiled can be scaled up exponentially with repeated rounds of pooling and splitting to enable single cell experiments with hundreds of thousands to millions of cells.
ScaleBio's products enable a unique combination of increased throughput, multi-omics support, cost-effective library preparation, and high-quality data.
Scalable sample indexing
Enables higher levels of sample multiplexing per experiment with ability to store sample for future experiments
Highly efficient chemistries
Enables the generation of complex single cell libraries for deeper profiling of cells
Scalable cell throughput
Enables profiling of hundreds of thousands to millions of cells per experiment
Cost effective library preparation
Enables large and complex library generation at low cost per sample and cell
Multi-modal application support
Enables Genomic, Proteomic, Epigenomic, Transcriptomic and Multi-omics applications
Instrument agnostic and compatible
Compatible with and independent of on-market single cell droplet and well based systems
Watch how ScaleBio's technology and methods have been used in single cell analysis of mammalian organogenesis
ScaleBio’s library preparation technology enables the generation of larger and more complex single cell libraries, leading to improved visualization and increased insights from high-dimensional single-cell data.
about our technology
Single-Cell Profiling and Analysis
PRODUCTS : EPIGENETIC PROFILING
ScaleBio’s single-cell chromatin accessibility kit enables users to profile up to 300,000 nuclei in a single experiment, decreasing the cost per cell while maintaining data quality. The streamlined workflow integrates tagmentation and sample indexing, enabling users to profile up to 24 samples simultaneously. Please contact us below for more information or to request a quote.
PRODUCTS : WHAT'S NEXT
ScaleBio is quickly following-on with additional single-cell combinatorial indexing applications and workflows, including single-cell transcriptome profiling for higher throughput experiments. Our scRNA kit will enable the price point needed for rigorous perturbation studies, large patient cohorts, and more.
If interested in participating in our RNA early access programs or learning more about planned applications for high-parameter protein and methylation profiling solutions, please reach out below.
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Join us in unlocking the potential for single cell analysis in biological discovery.
Companies don't do great science, people do. ScaleBio has patented technology, a strong co-founding team, leading scientific advisors, and value-add investors with a proven track record in building successful life sciences technology companies.
We are building a diverse, multi-disciplinary team of scientists, engineers, technologists, and bioinformaticians that share a passion for unlocking the potential of single cell sequencing. Are you interested in joining us?
Publications and Resources
Representative publications citing single cell split-pool barcoding and combinatorial indexing methods.
ScaleBio was founded by a multidisciplinary team of scientists and technologists, with expertise in next generation sequencing, genomics and proteomics and bioinformatics, that share a mission for unlocking the potential of single cell analysis at scale.
Get in touch for more information about our technology or products.
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