How it works
ScaleBio’s combinatorial indexing based single-cell profiling and analysis solutions enable increased levels of sample multiplexing, increased cell throughput, and support for a broad range of genomic, epigenomic, transcriptomic, proteogenomic, multi-omic applications.
Combinatorial indexing technology and methods leverage the cell as a compartment, enabling an accessible, instrument free, easy to adopt 2 day single-cell (and nuclei) profiling workflow:
1. Permeabilized cells are distributed in a multi-well plate where barcoded probes tag cellular analytes with a barcode.
2. These barcoded cells are pooled, then randomly re-distributed for another round of barcoding or indexing.
3. This process is completed when the desired barcode space has been achieved.
The number of cells or nuclei profiled can be scaled up exponentially with repeated rounds of pooling and splitting to enable single-cell experiments with up to 96 samples and 100,00 to millions of cells, at a fraction of the cost of on-market single-cell isolation instruments.
ScaleBio single-cell profiling and analysis solutions enable a unique combination of increased throughput, multi-omics support, high data quality and lower overall prep and sequencing costs.
Scalable sample indexing
Enables sample multiplexing up to 96 samples per experiment, with ability to fix, store and transport samples.
Highly efficient chemistries
Enables the construction of complex single-cell libraries that result in exceptional data quality.
Scalable cell throughput
Enables profiling of hundreds of thousands to millions of cells or nuclei per experiment with low multiplet rates.
Cost effective library preparation
Enables library construction at a fraction of the cost per sample and cell cost relative to on-market systems.
Broad application support
Enables Genomic, Proteomic, Epigenomic, Transcriptomic and Multi-omics profiling applications.
Accessible technology and workflow
Compatible with and independent of on-market single cell droplet and well based single-cell systems.
Learn about ScaleBio technology and how it's workflow can empower researchers to complete sample to insight solutions.
ScaleBio’s library preparation technology enables the generation of larger and more complex single cell libraries, leading to improved visualization and increased insights from high-dimensional single-cell data.
about our technology
ScaleBio Product Offerings
ScaleBio's scRNAseq 3' gene expression library preparation kit and analysis software enables the indexing of up to 96 samples and up to 125,000 of cells in a single experiment in support of larger and more complex single-cell studies. ScaleBio's scRNAseq kit is compatible with mouse and human cells lines, PBMCs and nuclei sample types.
ScaleBio’s scATACseq chromatin accessibility library preparation pre-indexing kit and analysis software enables the indexing up to 24 samples and profiling of up to 300,000 nuclei in a single experiment, support larger and more complex single-cell studies. ScaleBio's scATACseq solution has been demonstrated on a broad range of sample types and applications.
OTHER PLANNED SOLUTIONS
ScaleBio is developing Methylation, Protein, and Multiomic profiling applications to enable the discovery more biology at scale. In parallel we are working to demonstrate our existing scRNAseq and scATACseq products in key application areas. Stay tuned for more updates on these exciting new planned products or reach out to us if you are interested in collaborating or participating in our early access programs.
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Explore our available opportunities
Join us in unlocking the potential for single-cell analysis at scale and discover biology.
Companies don't do great science, people do. ScaleBio has patented disruptive technologies, a strong founding team of inventors, and a talented multidisciplinary team with a shared mission to enable researchers to unlock the potential for biological discovery at single-cell resolution. We are building a diverse, multi-disciplinary team of scientists, engineers, technologists, and bioinformaticians that share our mission for making our technology and methods more accessible to the broader research community. Are you interested in joining us?
Publications and Resources
Recent publications citing ScaleBio's combinatorial indexing technology and products and related resources.
ScaleBio was founded by a multidisciplinary team of scientists and technologists, with expertise in next generation sequencing, genomics and proteomics and bioinformatics, that share a mission for unlocking the potential of single cell analysis at scale.
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