Scale Bio Data Sets
Welcome! Here you can access representative data sets featuring Scale Bio's single-cell library preparation kits. You will find a top-level run report, as well as data output files processed through our Scale Bio Seq Suite pipeline, which can be reviewed and used for further processing. For an overview of the specifics of the pipeline, please refer to the bioinformatics handbook links under Product Information.
If you would like to request raw data access, please Contact Us.
Single Cell CRISPR Guide Enrichment Data Sets
Single Cell Methylation Data Sets
Single cell RNA data sets
Single Cell RNA Kit with Extended Throughput - PBMCs
Product Information:
Scale Bio Single Cell RNA Sequencing Kit v1.1
Scale Bio Single Cell RNA Extended Throughput Kit v1.1
Scale Bio Seq Suite RNA Pipeline version 1.1
Sample Information:
Sample format: cryopreserved
Species: Human
Sample/Cell Type: PBMCs
Cells in dataset: 600,000
Sequencing Information:
Sequencing platform: NovaSeq 6000
Flow Cell: S4
Mean passing reads per cell: 7,344
- HTML Report - this report is a high-level summary report for all samples.
- QC Summary Reports - Scale Bio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.
Single Cell RNA with Extended Throughput - Mouse Tissue Atlas
Product Information:
Scale Bio Single Cell RNA Sequencing Kit v1.1
Scale Bio Single Cell RNA Extended Throughput Kit v1.1
Scale Bio Seq Suite RNA Pipeline version 1.5.0-beta2
Scale Bio Seq Suite RNA Handbook_RevC
Scale Bio GitHub Repository - RNA Pipeline
Sample Information:
Sample format: flash frozen mouse tissue
Species: Mouse
Sample/Cell Type: Tissue derived nuclei (brain, lung, kidney, liver)
Cells in dataset: 468,081
Sequencing Information:
Sequencing platform: NovaSeq 6000
Flow Cell: S4
Mean passing reads per cell: 14,827
3378 (Lung), 18,693 (Liver), 5,360 (Kidney), 31,878 (Brain)
- HTML Report - this report is a high-level summary report for all samples.
- QC Summary Reports - Scale Bio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.
CRISPR Guide Enrichment Data Sets
CRISPR Guide Enrichment Kit - Cardiac Organoids
Product Information:
Scale Bio CRISPR Guide Enrichment Kit v1.1
Scale Bio Seq Suite: CRISPR v1.0.0
Scale Bio Seq Suite CRISPR Handbook_RevA
Scale Bio GitHub Repository - CRISPR Pipeline
Sample Information:
Sample format: cryopreserved
Species: Human
Sample/Cell Type: Cardiac Organoids
Cells in dataset: 47,000
Sequencing Information:
Sequencing platform: NovaSeq 6000
Flow Cell: S4
Mean passing reads per cell - cardiac organoids: 3,493
- HTML Report - this report is a high-level summary report for all samples.
- QC Summary Reports - Scale Bio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.
Single Cell Methylation Data Sets
Single Cell Methylation - PBMCs
Product Information:
Scale Bio Methylation Kit v1.0
Scale Bio Seq Suite: scMethylation v1.0.0
Top-level run metric detail summary
Scale Bio Seq Suite Methylation Handbook_RevA
Scale Bio GitHub Repository - Methylation Pipeline
Sample Information:
Sample format: cryopreserved
Species: Human, two separate male donors
Sample/Cell Type: PBMCs
Cells in dataset: 4,422
PBMC Donor 1 (S1): 2,373
PBMC Donor 2 (S2): 2,049
Sequencing Information:
Sequencing platform: NovaSeq 6000
Flow Cell: S4
Mean passing reads per cell:
PBMC Donor 1: 5,509,481
PBMC Donor 2: 4,791,442
- HTML Report - this report is a high-level summary report for all samples.
- QC Summary Reports - Scale Bio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.
Single Cell Methylation - Glioblastoma 8K
Product Information:
Scale Bio Methylation Kit v1.0
Scale Bio Seq Suite Methylation v1.0.1
Top-level run metric detail summary
Scale Bio Seq Suite Methylation Handbook_RevA
Scale Bio GitHub Repository - Methylation Pipeline
Sample Information:
Sample format: snap frozen tissue
Species: Human
Sample/Cell Type: Human Brain, Glioblastoma nuclei
Cells in dataset: 8,094
Sequencing Information:
Sequencing platform: NovaSeq 6000
Flow Cell: S4
Mean passing reads per cell: 1,877,340
- HTML Report - this report is a high-level summary report for all samples.
- QC Summary Reports - Scale Bio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.