ScaleBio Data Sets

Welcome! Here you can access representative data sets featuring ScaleBio's single-cell library preparation kits.  You will find a top-level run report, as well as data output files processed through our ScaleBio Seq Suite pipeline, which can be reviewed and used for further processing.  For an overview of the specifics of the pipeline, please refer to the bioinformatics handbook links under Product Information.

If you would like to request raw data access, please Contact Us.

 

Single Cell RNA Data Sets

Single Cell CRISPR Guide Enrichment Data Sets

Single Cell Methylation Data Sets

 

Single Cell RNA Data Sets

Single Cell RNA Kit with Extended Throughput - PBMCs

Product Information:

ScaleBio Single Cell RNA Sequencing Kit v1.1

ScaleBio Single Cell RNA Extended Throughput Kit v1.1

ScaleBio Seq Suite RNA Pipeline version 1.1

ScaleBio Seq Suite RNA Handbook_RevC

ScaleBio GitHub Repository - RNA Pipeline

Sample Information:

Sample format: cryopreserved

Species: Human

Sample/Cell Type: PBMCs

Cells in dataset: 600,000

Sequencing Information:

Sequencing platform: NovaSeq 6000

Flow Cell: S4

Mean passing reads per cell: 7,344

  • HTML Report - this report is a high-level summary report for all samples.
  • QC Summary Reports - ScaleBio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.

Single Cell RNA with Extended Throughput - Mouse Tissue Atlas

Product Information:

ScaleBio Single Cell RNA Sequencing Kit v1.1

Scale Bio Single Cell RNA Extended Throughput Kit v1.1

ScaleBio Seq Suite RNA Pipeline version 1.5.0-beta2

ScaleBio Seq Suite RNA Handbook_RevC

ScaleBio GitHub Repository - RNA Pipeline

 

 

Sample Information:

Sample format: flash frozen mouse tissue

Species: Mouse

Sample/Cell Type: Tissue derived nuclei (brain, lung, kidney, liver)

Cells in dataset: 468,081

 

Sequencing Information:

Sequencing platform: NovaSeq 6000

Flow Cell: S4

Mean passing reads per cell: 14,827

3378 (Lung), 18,693 (Liver), 5,360 (Kidney), 31,878 (Brain)

 

  • HTML Report - this report is a high-level summary report for all samples.
  • QC Summary Reports - ScaleBio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.

CRISPR Guide Enrichment Data Sets

CRISPR Guide Enrichment Kit - Cardiac Organoids

Product Information:

ScaleBio CRISPR Guide Enrichment Kit v1.1

ScaleBio Seq Suite: CRISPR v1.0.0

ScaleBio Seq Suite CRISPR Handbook_RevA 

ScaleBio GitHub Repository - CRISPR Pipeline

 

Sample Information:

Sample format: cryopreserved

Species: Human

Sample/Cell Type: Cardiac Organoids

Cells in dataset: 47,000

Sequencing Information:

Sequencing platform: NovaSeq 6000

Flow Cell: S4

Mean passing reads per cell - cardiac organoids: 3,493

  • HTML Report - this report is a high-level summary report for all samples.
  • QC Summary Reports - ScaleBio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.

Single Cell Methylation Data Sets

Single Cell Methylation - PBMCs

Product Information:

ScaleBio Methylation Kit v1.0

ScaleBio Seq Suite: scMethylation v1.0.0

Top-level run metric detail summary

ScaleBio Seq Suite Methylation Handbook_RevA

ScaleBio GitHub Repository - Methylation Pipeline

 

Sample Information:

Sample format: cryopreserved

Species: Human, two separate male donors

Sample/Cell Type: PBMCs

Cells in dataset: 4,422

PBMC Donor 1 (S1): 2,373

PBMC Donor 2 (S2): 2,049

Sequencing Information:

Sequencing platform: NovaSeq 6000

Flow Cell: S4

Mean passing reads per cell:

PBMC Donor 1: 5,509,481

PBMC Donor 2: 4,791,442

  • HTML Report - this report is a high-level summary report for all samples.
  • QC Summary Reports - ScaleBio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.

Single Cell Methylation - Glioblastoma 8K

Product Information:

ScaleBio Methylation Kit v1.0

ScaleBio Seq Suite Methylation v1.0.1

Top-level run metric detail summary

ScaleBio Seq Suite Methylation Handbook_RevA

ScaleBio GitHub Repository - Methylation Pipeline

 

Sample Information:

Sample format: snap frozen tissue

Species: Human

Sample/Cell Type: Human Brain, Glioblastoma nuclei

Cells in dataset: 8,094

 

Sequencing Information:

Sequencing platform: NovaSeq 6000

Flow Cell: S4

Mean passing reads per cell: 1,877,340

  • HTML Report - this report is a high-level summary report for all samples.
  • QC Summary Reports - ScaleBio Suite Pipeline - This folder contains the analyzed run data for each sample in the experiment.